Using the wrong solution type is another frequent error: substituting tap water, saline, or an unverified liquid for pharmaceutical-grade bacteriostatic or sterile water introduces contamination risk regardless of how carefully everything else is handled
Nat Commun (2018) 9(1):5337
The authors also thank E
In some cases, the pressure on the nerve wears away its protective outer layer (myelin sheath), which may cause pain signals to travel along the nerve
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Other liquids regular tap water, distilled water, saline, or even sterile water without preservative fail one or more of these criteria